Butaprost

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Butaprost

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	69685-22-9
包装:	5mg (12.24 mM * 1 mL in Methyl acetate)
市场价:	11680元

Butaprost 是一种选择性前列腺素 E 受体 (EP2) 激动剂，对鼠类EP2受体的EC₅₀值为 33 nM，K_i值为 2.4 μM。Butaprost 对鼠类 EP1，EP3 和 EP4 受体的活性较低。Butaprost 通过阻碍TGF-β/Smad2信号传导来减轻纤维化。

生物活性

Butaprost is a selectiveprostaglandin E receptor (EP2)agonist with anEC₅₀of 33 nM and aK_iof 2.4 μM formurine EP2 receptor. Butaprost is less activity against murine EP1, EP3 and EP4 receptors. Butaprost attenuates fibrosis by hamperingTGF-β/Smad2signalling^[1]^[2]^[3].

体外研究
(In Vitro)

Butaprost (1-100 nM; 0.5-24 hours) induces about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells in a dose- and time-dependent. Butaprost upregulated Nur77 gene expression through the PKC pathway^[1].
Butaprost (50 μM; 24 hours) reduces TGF-β-induced fibronectin (FN) expression, Smad2 phosphorylation and epithelial-mesenchymal transition in Madin-Darby Canine Kidney (MDCK) cells^[2].

Western Blot Analysis^[1]

Cell Line:	HEK 293/EBNA cells stably expressing the human EP2 receptor (hEP2-HEK 293/EBNA cells)
Concentration:	1 nM, 10 nM, 100 nM
Incubation Time:	0.5 hours, 1 hours, 6 hours, 24 hours
Result:	Induced about a five-fold upregulation of Nur77 mRNA expression in hEP2-HEK293/EBNA cells.

体内研究
(In Vivo)

Butaprost (1-4 mg/kg; intraperitoneal injection; twice daily; for 7 days) treatment attenuates the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery, as illustrated by a reduction in the gene and protein expression of α-smooth muscle actin, fibronectin and collagen 1A1^[2].

Animal Model:	Male C57BL/6 mice (8 weeks of age; 21 g) bearing unilateral ureteral obstruction surgery^[2]
Dosage:	1 mg/kg, 2 mg/kg, 4 mg/kg
Administration:	Intraperitoneal injection; twice daily; for 7 days
Result:	Attenuated the development of fibrosis in mice that underwent unilateral ureteral obstruction surgery.

分子量

408.57

性状

Liquid

Formula

C₂₄H₄₀O₅

CAS 号

69685-22-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Solution, -20°C, 2 years