Silybin 是一种从草蓟 (Silybum marianum) 种子中分离出来的黄素寡糖。Silybin 诱导凋亡 (apoptosis)，并具有保肝，抗氧化，抗炎，抗癌活性。
| 生物活性 | Silybin is a flavonolignan isolated from milk thistle (Silybum marianum) seeds. Silybin inducesapoptosisand exhibits hepatoprotective, antioxidant, anti-inflammatory, anti-cancer activity[1][2]. |
体外研究
(In Vitro) | Silybin (0-200 mM; for 72 hours) has growth inhibition in a time- and dose-dependent manner[1].
Silybin (68 μM; for 72 hours) induces apoptosis and increases the cells in G1-phase of ~22%[1].
Silybin (68 μM; for 72 hours) induces AKT activity inhibition[1].
Cell Viability Assay[1] | Cell Line: | HepG2 cell growth | | Concentration: | 0-200 mM | | Incubation Time: | For 72 hours | | Result: | Had growth inhibition in a time- and dose-dependent manner with an IC50of 68 μM. |
Apoptosis Analysis[1] | Cell Line: | HepG2 cell growth | | Concentration: | 68 μM | | Incubation Time: | For 72 hours | | Result: | Induced apoptosis in a higher number of cells (60%) when compared to untreated cells. |
Cell Cycle Analysis[1] | Cell Line: | HepG2 cell growth | | Concentration: | 68 μM | | Incubation Time: | For 72 hours | | Result: | Increased the cells in G1-phase of ~22% and decreased of 47% the cells in S-phase. |
Western Blot Analysis[1] | Cell Line: | HepG2 cell growth | | Concentration: | 68 μM | | Incubation Time: | For 72 hours | | Result: | Induced AKT activity inhibition. |
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体内研究
(In Vivo) | Silybin (50, 100 mg/kg/day; given intragastrically for the last 4 weeks) significantly lowers both serum and hepatic lipid accumulation[2].
| Animal Model: | Male C57BL/6J mice (6-8 weeks old) with nonalcoholic fatty liver disease (NAFLD)[2] | | Dosage: | 50, 100 mg/kg | | Administration: | Given intragastrically; daily; for the last 4 weeks | | Result: | Significantly lowered both serum and hepatic lipid accumulation. |
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| 来源 | - Plants
- Leguminosae
- Glycine soya
- Plants
- Compositae
- Silybum marianum(L.) Gaertn.
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : 25 mg/mL(51.82 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.0728 mL | 10.3640 mL | 20.7280 mL | | 5 mM | 0.4146 mL | 2.0728 mL | 4.1456 mL | | 10 mM | 0.2073 mL | 1.0364 mL | 2.0728 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.5 mg/mL (5.18 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (5.18 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
*以上所有助溶剂都可在本网站选购。 |
