SP-100030

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SP-100030

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

154563-54-9

SP-100030 是一种有效的NF-κB和激活蛋白-1(AP-1)双抑制剂 (IC₅₀分别为 50 和 50 nM)。SP-10003 抑制 Jurkat和其他T细胞系产生的 IL-2、IL-8 和 TNF-α 的产生。SP-100030 降低小鼠胶原性关节炎 (CIA)。

生物活性

SP-100030 is a potentNF-κBandactivator protein-1 (AP-1)double inhibitor (IC₅₀s=50 and 50 nM, respectively). SP-100030 inhibitsIL-2,IL-8, andTNF-alphaproduction in Jurkat and other T cell lines. SP-100030 decreases murine collagen-induced arthritis (CIA)^[1]^[2].

体外研究
(In Vitro)

SP-100030 blocks the production of IL-2 and IL-8 in Jurkat T-cells at the same concentrations as seen in the luciferase assay (IC₅₀≈30 nM)^[1].
ELISA and RT-PCR confirmed that IL-2, IL-8, and TNF-alpha production by activated Jurkat and other T cell lines were inhibited by SP100030^[2].

体内研究
(In Vivo)

SP100030 (10 mg/kg/day; i.p.; day 21 to day 34) significantly decreased arthritis severity^[2].

Animal Model:	Six- to 8-wk-old male DBA/1J mice (immunized with type II collagen)^[2]
Dosage:	10 mg/kg
Administration:	I.p.; daily, starting at day 21 to the end of the study on day 34
Result:	Significantly decreased arthritis scores compared with controls; Histologic evaluation of the paws from mice treated from day 20 to 34 showed a trend toward decreased inflammation.

分子量

437.65

性状

Solid

Formula

C₁₄H₅ClF₉N₃O

CAS 号

154563-54-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

DMSO : 50 mg/mL(114.25 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.2849 mL	11.4247 mL	22.8493 mL
5 mM	0.4570 mL	2.2849 mL	4.5699 mL
10 mM	0.2285 mL	1.1425 mL	2.2849 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。