C2 Ceramide

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C2 Ceramide

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

3102-57-6

Ceramide 2

C2 Ceramide (Ceramide 2) 是一种角质层的主要脂质，也是一种蛋白质磷酸酶 1 (PP1) 的激活剂。C2 Ceramide 还可激活PP2A和神经酰胺激活的蛋白磷酸酶 (CAPP)。C2 Ceramide 可诱导细胞分，自噬autophagy和凋亡 (apoptosis)，抑制线粒体呼吸链复合物 III (mitochondrial respiratory chain complex III)。C2 Ceramide 是一种皮肤调理剂，可保护表皮屏障免受水分流失。

生物活性

C2 Ceramide (Ceramide 2) is the main lipid of the stratum corneum and aproteinphosphatase1 (PP1)activator. C2 Ceramide activatesPP2Aandceramide-activated proteinphosphatase(CAPP). C2 Ceramide induces cells differentiation,autophagyandapoptosis, inhibitsmitochondrial respiratory chain complex III. C2 Ceramide is also a skin conditioning agent that protects the epidermal barrier from water loss^[1]^[2]^[3]^[4]^[5].

IC₅₀& Target

Protein phosphatase 1^[2]
PP2A^[4]
Ceramide-activated protein phosphatase (CAPP)^[4]
Apoptosis^[1]
Mitochondrial respiratory chain complex III^[1]

体外研究
(In Vitro)

C2 Ceramide (5 nM-200 μM; 24 hours; primary mouse osteoblasts) treatment (≤500 nM) promots osteoblast viability, whilst concentrations ≥2 μM significantly reduces osteoblast viability in a dose- and time-dependent manner^[1].
C2 Ceramide increases cytoplasmic histone-associated DNA fragments by 5.7- and 11.2-fold at 50 μM and 100 μM C2 Ceramide concentrations respectively in osteoblasts. At these higher concentrations, C2 Ceramide is a potent inducer of apoptosis in osteoblasts^[1].
C2 Ceramide up-regulates mRNA expression of angiogenic genes in human dental pulp cells (HDPCs) and increases the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA shows opposite effects. Human dental pulp cells (HDPCs) increases levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix^[2].

Cell Viability Assay^[1]

Cell Line:	Primary mouse osteoblasts
Concentration:	5 nM-200 μM
Incubation Time:	24 hours
Result:	Murine osteoblasts demonstrated a dose-dependent increase in their survival rate when exposed to low concentrations of 5-500 n M. Increasing concentrations of 20-200μM caused a dose-dependent decrease in mitochondrial succinate dehydrogenase activity and osteoblast survival.

体内研究
(In Vivo)

The PP1 activator C2 Ceramide increases alkaline phosphatase activity, mineralizes nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibits odontoblastic differentiation^[2].

分子量

341.53

性状

Solid

Formula

C₂₀H₃₉NO₃

CAS 号

3102-57-6

中文名称

C2 神经酰胺

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据

In Vitro:

DMSO : 20 mg/mL(58.56 mM;Need ultrasonic and warming)

Ethanol : 17 mg/mL(49.78 mM;Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.9280 mL	14.6400 mL	29.2800 mL
5 mM	0.5856 mL	2.9280 mL	5.8560 mL
10 mM	0.2928 mL	1.4640 mL	2.9280 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。