Rubropunctatin 是一种从红曲霉发酵米(红霉米)提取物中分离出来的橙色的氮杂酮类色素。 Rubropunctatin 具有抗炎，免疫抑制和抗氧化作用，还具有抗肿瘤活性。
| 生物活性 | Rubropunctatin, an orange azaphilone pigment, is isolated from the extracts ofMonascus pilosus-fermented rice (red-mold rice). Rubropunctatin has anti-inflammatory, immunosuppressive and antioxidative effects, and also exhibits anti-tumor activity[1][2][3]. |
体外研究
(In Vitro) | Rubropunctatin (1.5-30 μM; 24 h) shows selective tumoricidal effect on the human gastric carcinoma BGC-823 cells and no significant toxicity toward normal epithelial cell GES-1[1].
Rubropunctatin (5-30 μM; 6-24 h) induces apoptosis in a dose- and time-dependent manner in BGC-823 cells[1].
Rubropunctatin (0.75-8.0 μg/ml) exhibits DDPH radical scavenging activity, inhibition of super oxide radical generation and ferric reducing activity[2].
Cell Viability Assay[1] | Cell Line: | BGC-823 and GES-1cells | | Concentration: | 1.5, 3, 6, 12, 15, 18, 30 μM | | Incubation Time: | 24 hours | | Result: | Decreased the viability of BGC-823 cells with an IC50of 12.57 μM for 24 h.
Did not show obvious cytotoxic effects on the normal cells. |
Cell Cycle Analysis[1] | Cell Line: | BGC-823 cells | | Concentration: | 0, 5, 10, 30 μM | | Incubation Time: | 0, 6, 12, 24 hours | | Result: | Increased the percentage of cells in sub-G1 phase in a dose- and time-dependent manner.
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体内研究
(In Vivo) | Rubropunctatin (8-32 mg/kg; i.v. for 5 times) has anti-tumor effect mice[1].
Rubropunctamine inhibits 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced inflammation in mice, with an ID50of 0.11 mg/ear[3].
| Animal Model: | Male nude mice (5 weeks) are inoculated with BGC-823 cells[1] | | Dosage: | 8, 32 mg/kg | | Administration: | I.v. five times (day 1st, 4th, 7th, 10th, and 13th) | | Result: | Diminished the tumor volume by 11.1% (8 mg/kg) and 24.2% (32 mg/kg).
Reduced the tumor weight by 23.5% (8 mg/kg) and 37.7% (32 mg/kg).
No significant difference was observed on the body weight. |
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| 结构分类 | - Ketones, Aldehydes, Acids
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
