Citric acid monohydrate

立即咨询

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

5949-29-1

一水柠檬酸

Citric acid monohydrate 是一种天然防腐剂和食品酸味增强剂。Citric acid monohydrate诱导细胞凋亡 (apoptosis) 和细胞周期停滞在 G2/M 期和 S 期。Citric acid monohydrate 通过降低抗氧化酶活性引起肝脏的氧化损伤。Citric acid monohydrate 对小鼠造成肾毒性。

生物活性

Citric acid monohydrate is a natural preservative and food tartness enhancer. Citric acid monohydrate inducesapoptosisand cell cycle arrest at G2/M phase and S phase. Citric acid monohydrate cause oxidative damage of the liver by means of the decrease of antioxidative enzyme activities. Citric acid monohydrate causes renal toxicity in mice^[1]^[2]^[3].

IC₅₀& Target

Human Endogenous Metabolite

体外研究
(In Vitro)

Citric acid monohydrate (0-12.5 mM; 24 h) shows antiproliferative activity in a dose dependent manner^[3].
Citric acid monohydrate (12.5 mM; 72 h) induces apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner^[3].
Citric acid monohydrate (12.5 mM; 48 h) increases the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreases of BCL-2 and BCL-Xl^[3].

Cell Viability Assay^[3]

Cell Line:	HaCaT cells
Concentration:	0, 2.5, 5, 7.5, 10, 12.5 mM
Incubation Time:	24 h
Result:	Inhibited the cell viability in a dose dependent manner.

Cell Cycle Analysis^[3]

Cell Line:	HaCaT cells
Concentration:	12.5 mM
Incubation Time:	0, 12, 24, 48, 72 h
Result:	Induced apoptosis and cell cycle arrest at G2/M phase and S phase in a dosedependent manner.

Western Blot Analysis^[3]

Cell Line:	HaCaT cells
Concentration:	12.5 mM
Incubation Time:	12, 24, 48 h
Result:	Increased the expression of FAS, BAX, BID, AIF, EndoG, cytochrome c, PARP, GADD153, GRP78 and caspase-3, -8, -9, and decreased of BCL-2 and BCL-Xl.

体内研究
(In Vivo)

Citric acid monohydrate (120, 240, and 480 mg/kg; i.p.) significantly decreases GSH-Px activity and induces an increase in the MDA (malonyldialdehyde) levels in mouse liver^[1].
Citric acid monohydrate (120, 240, and 480 mg/kg; i.p.) induces apoptosis by increases caspase-3 activity in a dose-dependent manner in mouse hepatocytes^[1].
Citric acid monohydrate (120, 240, and 480 mg/kg; i.p.; weekly for 3 weeks) causes renal toxicity in mice^[2].

Animal Model:	20 g male Kunming mice^[2]
Dosage:	120, 240, 480 mg/kg
Administration:	I.p.; weekly for 3 weeks
Result:	T-SOD and GSH-Px activities in the treated groups decreased with increasing doses of citric acid, NOS activity tended to increase, and H2O2 and MDA contents gradually decreased.

Clinical Trial

分子量

210.14

性状

Solid

Formula

C₆H₁₀O₈

CAS 号

5949-29-1

中文名称

一水柠檬酸

结构分类

Ketones, Aldehydes, Acids

来源

Plants

Aspergillus niger

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)