Irisflorentin 是一种天然存在的异黄酮，是射干中丰富的一种活性成分。Irisflorentin 显着降低了诱导型一氧化氮合酶 (iNOS) 的转录和翻译水平以及NO产生。
| 生物活性 | Irisflorentin, a naturally occurring isoflavone, is an abundant active constituent inBelamcanda chinensis. Irisflorentin markedly reduces the transcriptional and translational levels of inducible nitric oxide synthase (iNOS) as well as the production of NO. Anti-inflammatory activity[1]. |
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体外研究
(In Vitro) | Irisflorentin does not affect the LPS-stimulated RAW 264.7 macrophages viability up to a concentration of 40 μM[1].
Irisflorentin (10-40 μM) inhibits the LPS-induced production of NO in a concentration-dependent manner without affecting the iNOS activity in RAW 264.7 macrophages[1].
Irisflorentin (10-40 μM) concentration dependently inhibits the LPS (10 ng/mL)-induced increase in iNOS mRNA expression in LPS stimulated RAW 264.7 macrophages. Irisflorentin (10-40 μM) concentration-dependently suppresses the production of TNF-α, IL-1β and IL-6 in LPS stimulated RAW 264.7 macrophages[1].
Irisflorentin (10-40 μM) concentration dependently inhibits the LPS (10 ng/mL)-induced increase in iNOS protein levels. Irisflorentin (10-40 μM) concentration-dependently suppresses LPS-induced p38 and ERK1/2 phosphorylation in a concentration-dependent manner, but slightly affects JNK phosphorylation[1].
RT-PCR[1] | Cell Line: | LPS-stimulated RAW 264.7 macrophages | | Concentration: | 10, 20, 40 μM | | Incubation Time: | | | Result: | Inhibited the LPS (10 ng/mL)-induced increase in iNOS mRNA expression.
Suppressed the production of TNF-α, IL-1β and IL-6. |
Western Blot Analysis[1] | Cell Line: | LPS-stimulated RAW 264.7 macrophages | | Concentration: | 10, 20, 40 μM | | Incubation Time: | | | Result: | Inhibited the LPS (10 ng/mL)-induced increase in iNOS protein levels.
Suppressed LPS-induced p38 and ERK1/2 phosphorylation, but slightly affected JNK phosphorylation. |
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| 来源 | - Plants
- Iridaceae
- Belamcanda chinensis(Linn.) Redouté
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : 50 mg/mL(129.42 mM;Need ultrasonic) 配制储备液 | 1 mM | 2.5883 mL | 12.9416 mL | 25.8833 mL | | 5 mM | 0.5177 mL | 2.5883 mL | 5.1767 mL | | 10 mM | 0.2588 mL | 1.2942 mL | 2.5883 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 In Vivo: 请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂： ——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；
以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶 1. 请依序添加每种溶剂： 10% DMSO 90%corn oil Solubility: ≥ 2.17 mg/mL (5.62 mM); Clear solution
此方案可获得 ≥ 2.17 mg/mL (5.62 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。 以 1 mL 工作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。
*以上所有助溶剂都可在本网站选购。 |
