Lysozyme chloride

立即咨询

Lysozyme chloride

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

9066-59-5

氯化溶菌酶

Lysozyme chloride 是一种杀菌酶，它能裂解革兰氏阳性细菌 (bacteria)。Lysozyme chloride 还可用于HIV感染和肺气肿的研究。

生物活性

Lysozyme chloride is a bactericidal enzyme, and it lyses gram-positivebacteria. Lysozyme chloride can also be used for the research ofHIVinfectionand pulmonary emphysema^[1]^[2]^[3].

体外研究
(In Vitro)

Lysozyme 是一种普遍存在的酶。鸡蛋是 Lysozyme 最丰富的来源，约占蛋白蛋白质的 3.4%。Lysozyme 是一种天然抗菌素，可水解细菌细胞壁肽聚糖层中 N-acetylmuramic acid 和 N-acetylglucosamine 之间的 β(1-4) 糖苷连接，导致细胞裂解。Lysozyme chloride 的杀菌作用主要局限于革兰氏阳性细菌，包括病原体，如Listeria monocytogenes和某些Clostridium，以及一些腐败生物，包括嗜热孢子形成细菌和某些酵母。革兰氏阴性菌由于其复杂的细胞壁结构而对 Lysozyme chloride 的作用更有抵抗力^[1]。
Lysozyme (1 mg/mL) chloride 降低透明质酸 (HA) 防止弹性蛋白酶损伤弹性纤维的能力^[3]。

体内研究
(In Vivo)

叙利亚仓鼠在给予弹性蛋白酶前暴露于雾化 Lysozyme (20 mg in 20 ml of water; 50 min) chloride 中导致气道扩大显著增加^[3]。

CAS 号

9066-59-5

中文名称

氯化溶菌酶

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility

In Vitro:

H₂O

Peptide Solubility and Storage Guidelines:

1. Calculate the length of the peptide.

2. Calculate the overall charge of the entire peptide according to the following table:

	Contents	Assign value
Acidic amino acid	Asp (D), Glu (E), and the C-terminal -COOH.	-1
Basic amino acid	Arg (R), Lys (K), His (H), and the N-terminal -NH₂	+1
Neutral amino acid	Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q)	0

3. Recommended solution:

Overall charge of peptide	Details
Negative (<0)	1. Try to dissolve the peptide in water first. 2. If water fails, add NH₄OH (<50 μL). 3. If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0)	1. Try to dissolve the peptide in water first. 2. If water fails, try dissolving the peptide in a 10%-30% acetic acid solution. 3. If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0)	1. Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first. 2. For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.