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Di-8-ANEPPS
本产品不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
Di-8-ANEPPS图片
CAS NO:157134-53-7

Di-8-ANEPPS 是萘基苯乙烯基电压敏感染料,它们的荧光激发和发射光谱会因电压的变化而变化。
生物活性

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm.

体外研究
(In Vitro)

A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the “center” signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1].

分子量

592.87

性状

Solid

Formula

C36H52N2O3S

CAS 号

157134-53-7

Emission (Em)
Em 590-620 nm Orange
Excitation (Ex)
Ex 495-570 nm Green
运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, protect from light

*该产品在溶液状态不稳定,建议您现用现配,即刻使用。

溶解性数据
In Vitro: 

DMSO : 1 mg/mL(1.69 mM;ultrasonic and warming and heat to 60℃)

配制储备液
浓度溶剂体积质量1 mg5 mg10 mg
1 mM1.6867 mL8.4336 mL16.8671 mL
5 mM---------
10 mM---------
*

请根据产品在不同溶剂中的溶解度,选择合适的溶剂配制储备液;该产品在溶液状态不稳定,建议您现用现配,即刻使用

 
 
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