NucPE1

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NucPE1

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

1404091-23-1

Nuclear Peroxy Emerald 1

NucPE1 (Nuclear Peroxy Emerald 1) 是核定位荧光过氧化氢探针, 其特异性定位于细胞核，没有附加的靶向部分。

生物活性

NucPE1 (Nuclear Peroxy Emerald 1) is a nuclear-localized fluorescent hydrogen peroxide that is specifically localized to cellular nuclei without appended targeting moieties.

体外研究
(In Vitro)

NucPE1 features two major visible region absorptions (λ_abs=468 nm, ε=27,300 M^-1cm^-1; λ_abs=490 nm, ε=26,000 M^-1cm^-1) and a weak emission (λ_em=530 nm, Φ=0.117). Reaction of NucPE1 with H₂O₂triggers a fluorescence increase upon its conversion to fluorophore NucPE1, which possesses one major absorption band at 505 nm (ε=19,100 M^-1cm^-1) with enhanced emission (λ_em=530 nm, Φ=0.626). NucPE1 selectively accumulates in the nuclei of a variety of mammalian cell lines as well as in whole model organisms likeC. clegans, where it can respond to subcellular changes in H₂O₂fluxes^[1].

体内研究
(In Vivo)

NucPE1 maintains the ability to selectively target nucleiin vivo. NucPE1 imaging reveals a reduction in nuclear H₂O₂levels in worms overexpressing sir-2.1 compared to wildtype congeners, supporting a link between this longevity-promoting sirtuin protein and enhanced regulation of nuclear ROS pools^[1].

分子量

483.36

性状

Solid

Formula

C₂₉H₃₀BNO₅

CAS 号

1404091-23-1

Emission (Em)

Em 450-495 nm Blue

Excitation (Ex)

Ex 450-495 nm Blue

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

溶解性数据

In Vitro:

DMSO : 25 mg/mL(51.72 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	2.0689 mL	10.3443 mL	20.6885 mL
5 mM	0.4138 mL	2.0689 mL	4.1377 mL
10 mM	0.2069 mL	1.0344 mL	2.0689 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light, stored under nitrogen)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： 10% DMSO 40%PEG300 5%Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (5.17 mM); Clear solution
此方案可获得 ≥ 2.5 mg/mL (5.17 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH2O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在本网站选购。