Pyranine

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Pyranine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

6358-69-6

溶剂绿7
HPTS
Solvent Green 7

Pyranine (HPTS; Solvent Green 7) 是一种 pH 敏感的荧光指示剂。Pyranine 是一类用于 Cu⁺离子的荧光化学传感器 (λex=450 nm，λem=510 nm)。

生物活性

Pyranine (HPTS; Solvent Green 7) is a pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu⁺ion(λex=450 nm, λem=510 nm)^[1]^[2]^[3].

体外研究
(In Vitro)

Pyranine is capable of discriminating ranges of cations from the Cu⁺ion, even in competing environment. Pyranine displays a rapid fluorescence response (t_1/2=1.66 min) towards the Cu⁺ion, and the micromolar detection limit enables the detection of the ion in environmental samples. The observed stoichiometry of complexation between Pyranine and Cu⁺is 2:1^[1].
The pH-sensitive fluorescent indicator Pyranine is studied to determine its usefulness as probes for the living cornea and anterior chamber. Adequate concentrations are reached in the cornea and anterior chamber after topical administration; measurements can be made by fluorophotometry for many hours. The pH is calculated by measuring the ratio of fluorescent intensity at two excitation wavelengths, I463/I404, a measurement which is dependent on pH but independent of the concentration of the fluorophore and other variables which can alter the intensity. In the rabbit eye, Pyranine in the cornea and anterior chamber is observed to undergo easily measurable changes in fluorescent ratios associated with lid closure and contact lens wear, indicating its sensitivity to mild changes in pH^[2].

分子量

524.39

性状

Solid

Formula

C₁₆H₇Na₃O₁₀S₃

CAS 号

6358-69-6

Emission (Em)

Em 495-570 nm Green

Excitation (Ex)

Ex 450-495 nm Blue

中文名称

溶剂绿7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

In Vitro:

H₂O : 100 mg/mL(190.70 mM;Need ultrasonic)

DMSO : 25 mg/mL(47.67 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	1.9070 mL	9.5349 mL	19.0698 mL
5 mM	0.3814 mL	1.9070 mL	3.8140 mL
10 mM	0.1907 mL	0.9535 mL	1.9070 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (sealed storage, away from moisture and light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 25 mg/mL (47.67 mM); Clear solution; Need ultrasonic and warming

*以上所有助溶剂都可在本网站选购。