DENV-IN-4 是一种有效的DENV抑制剂 (DENV EC50=4.79 μM, Vero CC50>100 μM, SI>20.9)。DENV-IN-4 可抑制 DENV2 的表达水平，具有浓度依赖性，并且能降低依赖性 RNA 聚合酶 (RNA-dependent RNA polymerase, RdRp) 的活性。DENV-IN-4 具有抗病毒作用。
| 生物活性 | DENV-IN-4 is a potentDENVinhibitor (DENV EC50=4.79 μM, Vero CC50>100 μM, SI>20.9). DENV-IN-4 can inhibit the expression level of DENV2 with concentration-dependence and reduce RNA-dependent RNA polymerase (RdRp) enzymatic activity. DENV-IN-4 has antiviral effect[1]. |
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体外研究
(In Vitro) | DENV-IN-4 (compound 15) (0.064-200 μM; 3 days) has antiviral effect and not related to its toxicity (DENV EC50=4.79 μM, Vero CC50>100, SI>20.9)[1].
DENV-IN-4 (3.125-50 μM in Vero, 3.75-30 μM in A549 and Huh-7; 48 hours) inhibits the expression level of DENV2 with concentration-dependence, and inhibition curves were smoother in Vero[1].
DENV-IN-4 (7.5 and 15 μM; 48 hours) can effectively inhibit DENV infection in Vero cells, and completely inhibited at 15 μM[1].
DENV-IN-4 (0-15 μM; 48 hours) inhibits DENV2 E protein production with good concentration-dependent relationship in Vero cells[1].
Cell Cytotoxicity Assay | Cell Line: | Vero[1] | | Concentration: | 0.064-200 μM | | Incubation Time: | 3 days | | Result: | Showed antiviral effect of DENV-IN-4 was not related to its toxicity (Vero EC50=4.79 μM, CC50>100, SI>20.9). |
RT-PCR | Cell Line: | Vero, A549 and Huh-7 cells[1] | | Concentration: | 3.125-50 μM in Vero, 3.75-30 μM in A549 and Huh-7 | | Incubation Time: | 48 hours | | Result: | Inhibited the expression level of DENV2 with concentration-dependence, and inhibition curves were smoother in Vero. |
Western Blot Analysis | Cell Line: | Vero[1] | | Concentration: | 7.5 and 15 μM | | Incubation Time: | 48 hours | | Result: | Effectively inhibited DENV infection and completely inhibited at 15 μM. |
Immunofluorescence | Cell Line: | Vero[1] | | Concentration: | 0-15 μM | | Incubation Time: | 48 hours | | Result: | Inhibited DENV2 E protein production with good concentration-dependent relationship. |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
