Neocryptotanshinone 是从丹参 (Salvia Miltiorrhiza) 分离得到的一种丹参二萜，通过抑制NF-κB和iNOS信号来抑制脂多糖 (LPS) 诱导的炎症。
| 生物活性 | Neocryptotanshinone, a fattyditerpenoidsfromSalvia Miltiorrhiza, inhibits lipopolysaccharide-induced inflammation by suppression ofNF-κBandiNOSsignaling pathways[1][2]. |
| IC50& Target | |
体外研究
(In Vitro) | Neocryptotanshinone exhibits anti-inflammatory effect by suppression of NF-κB and iNOS signaling pathways[1].
Neocryptotanshinone (10 μM and 20 μM, 24 h) inhibits LPS-induced iNOS protein expression in RAW264.7 cells[1].
Neocryptotanshinone (20 μM, 24 h) shows no obvious cytotoxic effect towards murine RAW264.7 macrophages[1].
Cell Viability Assay[1] | Cell Line: | RAW264.7 cells | | Concentration: | 5, 10 and 20 μM | | Incubation Time: | 24 hours | | Result: | Inhibited LPS-induced cell viability in a dose-dependent manner. |
Western Blot Analysis[1] | Cell Line: | RAW264.7 cells | | Concentration: | 20 μM | | Incubation Time: | 24 hours | | Result: | Inhibited LPS-induced activation of NF-κB pathway and down-regulated LPS-induced expression of p-NF-κB p65, p-IκBα and p-IKKβ. |
|
体内研究
(In Vivo) | Neocryptotanshinone causes reversals of decreased pain thresholds induced by MSU treatment after 30, 60, and 120 min S. miltiorrhiza Bunge extract treatment (contains single active components)[3].
| Animal Model: | MSU-induced pain model in male ICR mice (weighing 20-25 g)[2] | | Dosage: | 10, 25, 50 or 100 mg/kg | | Administration: | Oral gavage; for 30, 60, 120 min | | Result: | Inhibited inflammatory symptoms and nociceptive behaviors in a dose-dependent manner. |
|
| 分子量 | |
| 性状 | |
| Formula | |
| CAS 号 | |
| 中文名称 | |
| 结构分类 | - Quinones
- Naphthalene Quinones
|
| 来源 | - Plants
- Compositae
- Hemistepta lyrata
|
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | 4°C, protect from light *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light) |
| 溶解性数据 | In Vitro: DMSO : 100 mg/mL(318.09 mM;Need ultrasonic) 配制储备液 | 1 mM | 3.1809 mL | 15.9043 mL | 31.8086 mL | | 5 mM | 0.6362 mL | 3.1809 mL | 6.3617 mL | | 10 mM | 0.3181 mL | 1.5904 mL | 3.1809 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
