Leachianone A

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Leachianone A

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CAS NO:

97938-31-3

Leachianone A 从槐花中分离，具有抗疟疾，抗炎和细胞毒性作用。 Leachianone A 诱导细胞凋亡 (apoptosis)。

生物活性

Leachianone A, isolated fromRadix Sophorae, has anti-malarial, anti-inflammatory, and cytotoxic potent^[1]. Leachianone A inducesapoptosisinvolved both extrinsic and intrinsic pathways^[2].

体外研究
(In Vitro)

Leachianone A (0-20 μg/ml; 24-72 hours) exhibits a marked inhibition on the survival of HepG2 cells time- and dose-dependently manner, IC₅₀values are 6.9 μg/ml, 3.4 μg/ml and 2.8 μg/ml in cells with 24-, 48- and 72-hours treatment, respectively^[1].
Leachianone A (10-30 μg/ml; 48 hours) indicates that at low concentration of LA (10 μg/ml), a substantial amount of cells is primarily in the early phase of apoptosis, at higher concentrations, induces a shift of the cell population to late apoptotic/ necrotic stage^[1].
Leachianone A (10-30 μg/ml; 48 hours) decreases the precursor of caspase-3 in a dose-dependent manner, reduces the protein level of the pro-forms of upstream initiator caspases, caspases-8 and -9, decreases two downstream substrates, namely inhibitor of caspase-activated DNase(ICAD) and poly-ADP-ribose polymerase (PARP) in HepG2 cells^[1].

Cell Viability Assay^[1]

Cell Line:	HepG2 cells
Concentration:	0 μg/ml, 2 μg/ml, 4 μg/ml, 6 μg/ml, 8 μg/ml, 10 μg/ml, 12 μg/ml, 14 μg/ml, 16 μg/ml, 18 μg/ml, 20 μg/ml
Incubation Time:	24-72 hours
Result:	Inhibited HepG2 cells survival.

Apoptosis Analysis^[1]

Cell Line:	HepG2 cells
Concentration:	48 hours
Incubation Time:	10, 20, and 30 μg/ml
Result:	Induced the proportion of annexin V-stained cells in both the early and late apoptotic stage.

Western Blot Analysis^[1]

Cell Line:	HepG2 cells
Concentration:	48 hours
Incubation Time:	10, 20, and 30 μg/ml
Result:	Decreased the protein expression of caspase-3, caspases-8 and -9, reduced ICAD and PARP protein expression.

体内研究
(In Vivo)

Leachianone A (intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days) significantly diminishes the tumor volume by 17-54% in LA-treated nude mice, when compared with those solely given the vehicle^[1].

Animal Model:	Male nude mice with human hepatoma HepG2 cells^[1]
Dosage:	20 mg/kg; 30 mg/kg
Administration:	Intravenously injection; 20 mg/kg, 30 mg/kg; once daily; 30 days
Result:	Suppressed the tumor growth in vivo.

分子量

438.51

性状

Solid

Formula

C₂₆H₃₀O₆

CAS 号

97938-31-3

结构分类

Flavonoids
Flavonones

Phenols
Polyphenols

来源

Plants
Leguminosae
Sophora flavescens

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)