PI3K/Akt/mTOR-IN-2 是一种有效的PI3K/AKT/mTOR抑制剂。PI3K/Akt/mTOR-IN-2 具有抗癌作用，并对 MDA-MB-231 细胞具有选择性，IC50为 2.29 μM。PI3K/Akt/mTOR-IN-2 可诱导癌细胞周期阻滞和细胞凋亡 (apoptosis)。
| 生物活性 | PI3K/Akt/mTOR-IN-2 is aPI3K/AKT/mTORpathway inhibitor. PI3K/Akt/mTOR-IN-2 possess anti-cancer effects and selectivity against MDA-MB-231 cells withIC50value of 2.29 μM. PI3K/Akt/mTOR-IN-2 can inducecancercell cycle arrest andapoptosis[1]. |
| IC50& Target | IC50: 2.29 μM (PI3K/AKT/mTOR) in MDA-MB-231[1] |
体外研究
(In Vitro) | PI3K/Akt/mTOR-IN-2 (compound 23) (0.5 - 100 μM; 72 hours) exhibits effective anti-cancer activity with IC50s of 2.29 - 24.63 μM, of which, IC50in MDA-MB-231 is 2.29 μM[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24 hours) induces growth inhibition of MDA-MB-231 cells by cell cycle arrest at G0/G1[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24, 48 and 72 hours) induces apoptosis in MDA-MB-231 cells with both dose- and time-dependent manners[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 48 hours) increases the expression of Bax, and decreases the expression of Bcl-2 in MDA-MB-231 cells[1].
PI3K/Akt/mTOR-IN-2 (1 μM, 2 μM and 4 μM; 24 hours) induces mitochondria-dependent apoptosis in MDA-MB-231 cells through disruption of MMP, accumulation of ROS, depletion of GSH and elevation of intracellular Ca2+[1].
Cell Proliferation Assay | Cell Line: | PC3, BGC-823, A549, MCF-7, MDA-MB-231 cells and MCF-10A cells[1] | | Concentration: | 0.5 - 100 μM | | Incubation Time: | 72 hours | | Result: | Exhibited effective anti-cancer activity with IC50s of 2.29 - 24.63 μM, of which, IC50in MDA-MB-231 was 2.29 μM. |
Cell Cycle Analysis | Cell Line: | MDA-MB-231[1] | | Concentration: | 1 μM, 2 μM and 4 μM | | Incubation Time: | 24 hours | | Result: | Induced growth inhibition of MDA-MB-231 cells by cell cycle arrest at G0/G1. |
Apoptosis Analysis | Cell Line: | MDA-MB-231[1] | | Concentration: | 1 μM, 2 μM and 4 μM | | Incubation Time: | 24, 48 and 72 hours | | Result: | Induced apoptosis in MDA-MB-231 cells with both dose- and time-dependent manners. |
Western Blot Analysis | Cell Line: | MDA-MB-231[1] | | Concentration: | 1 μM, 2 μM and 4 μM | | Incubation Time: | 48 hours | | Result: | Increased the expression of Bax, and decreased the expression of Bcl-2 |
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| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | | Powder | -20°C | 3 years | | 4°C | 2 years | | In solvent | -80°C | 6 months | | -20°C | 1 month |
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| 溶解性数据 | In Vitro: DMSO : ≥ 100 mg/mL(350.52 mM) *"≥" means soluble, but saturation unknown. 配制储备液 | 1 mM | 3.5052 mL | 17.5260 mL | 35.0521 mL | | 5 mM | 0.7010 mL | 3.5052 mL | 7.0104 mL | | 10 mM | 0.3505 mL | 1.7526 mL | 3.5052 mL |
*请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。 |
