Stepharine

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Stepharine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

2810-21-1

Stepharine 是天然的生物碱，可直接靶向TLR4(TLR4 抑制剂)，结合 TLR4/MD2 复合体。Stepharine 具有抗衰老、抗病毒和抗高血压等活性。

生物活性

Stepharine, an natural alkaloid, directly interactes withTLR4and binds to the TLR4/MD2 complex (TLR4 inhibitor). Stepharine possessesanti-aging, anti-viral and anti-hypertensive effects^[1].

体外研究
(In Vitro)

Stepharine (10, 30 μM) substantially inhibits nitric oxide (NO) release as well as the mRNA and protein expression of pro-inflammatory mediators [inducible nitric oxide synthase, interleukin (IL)-6, tumor necrosis factor (TNF)-α, IL-1β] in LPS-activated BV-2 cells^[1].
Stepharine (10, 30 μM) inhibits LPS-induced increase of TLR4 expression, IκBα phosphorylation, and NF-κB p65 nuclear translocation^[1].
Stepharine exhibits neuroprotective effects on SH-SY5Y cells cultured with LPS-treated conditioned medium. Stepharine has also shown to inhibit cholinesterase in vitro^[1].

体内研究
(In Vivo)

Stepharine (500 μg/kg) inhibited NeuN⁺cells loss and Iba-1⁺cells increase in the MCAO ischemic cortex^[1].

Animal Model:	Middle cerebral artery occlusion (MCAO) rat model^[1].
Dosage:	500 μg/kg (20 μL).
Administration:	Intranasally injected into rats 1h after MCAO.
Result:	Attenuated the neuronal loss induced by MCAO (MCAO+ stepharine) group.

分子量

297.35

性状

Solid

Formula

C₁₈H₁₉NO₃

CAS 号

2810-21-1

结构分类

Alkaloids
Isoquinoline Alkaloids

来源

Plants
other families

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据

In Vitro:

DMSO : 25 mg/mL(84.08 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	3.3630 mL	16.8152 mL	33.6304 mL
5 mM	0.6726 mL	3.3630 mL	6.7261 mL
10 mM	0.3363 mL	1.6815 mL	3.3630 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。