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WST-8
本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
WST-8图片
CAS NO:193149-74-5
包装与价格：
包装价格(元)
10mM (in 1mL Water)询价
25mg询价
50mg询价
100mg询价
200mg询价

WST-8 是一种水溶性四唑鎓染料，与传统的基于 MTS 的测定相比，WST-8 提高了基于 WST-8 的测定的灵敏度。
Cas No.193149-74-5
别名2-(2-甲氧基-4-硝基苯)-3-(4-硝基苯)-5-(2,4-二磺基苯)-2H-四氮唑单钠盐
化学名2-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-5-sulfobenzenesulfonate, sodium salt
Canonical SMILESCOC1=C([N+]2=NC(C3=C(S(=O)([O-])=O)C=C(S(=O)(O)=O)C=C3)=NN2C4=CC=C([N+]([O-])=O)C=C4)C=CC([N+]([O-])=O)=C1.[Na+]
分子式C20H14N6NaO11S2+
分子量600.47
溶解度DMSO: 1 mg/ml,PBS (pH 7.2): 5 mg/ml
储存条件Store at -20℃,protect from light
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
文献引用
产品描述

WST-8 is a water-soluble tetrazolium dye, WST-8 enhances sensitivity of the WST-8-based assay over the conventional MTS-based assay.

The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×105 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line[1].

Reference：
[1]. Tiwari K, et al. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54.

 
 
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