L-SelenoMethionine

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L-SelenoMethionine

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

3211-76-5

L-硒代蛋氨酸

L-SelenoMethionine 是 Selenomethionine 的 L-异构体，是硒的主要天然食物形式。L-SelenoMethionine 是一种癌症化学预防剂，可以通过饮食补充降低癌症的发生率并诱导癌细胞凋亡。L-SelenoMethionine 还可以增加谷胱甘肽过氧化物酶的表达。

生物活性

L-SelenoMethionine, an L-isomer of Selenomethionine, is a major natural food-form of selenium. L-SelenoMethionin is acancerchemopreventive agent that can reducecancerincidence by dietary supplementation and induceapoptosisofcancercells. L-SelenoMethionine also can increase expression ofglutathione peroxidase^[1]^[2]^[3].

IC₅₀& Target

Microbial Metabolite

体外研究
(In Vitro)

L-SelenoMethionine (1-500 μM; 24-72 h) is selectively inhibits the growth of prostate cancer cells, compared with normal cells^[2].
L-SelenoMethionine (500 μM; 48 h) induce apoptosis in prostate cancer cells^[2].
L-SelenoMethionine (500 μM; 48 h) causes an increase in arrest in the G2-M phase of the cell cycle selectively in prostate cancer cells^[2].
L-SelenoMethionine (5 μM; 24 h) mitigates gene expression associated with the cellular stress response from 10 cGy irradiation^[4].

Cell Viability Assay^[2]

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	1, 5, 10, 50, 100, 500 μM
Incubation Time:	24, 48, 72 hours
Result:	Inhibited the growth of prostate cancer cells, with a lower IC₅₀(1-90 μM) at 72 h than normal prostate cells (>500 μM).

Apoptosis Analysis^[2]

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	500 μM
Incubation Time:	48 hours
Result:	Exhibited the highest level of DNA condensation in androgen-responsive LNCaP carcinoma cells, followed by PC-3 and DU145 cells. Exhibited the nicked-end DNA labeling in prostate cancer cells. Promoted the PARP cleavage in prostate cancer cells.

Cell Cycle Analysis^[2]

Cell Line:	Prostate cancer cells (LNCaP, PC-3, and DU145) and normal prostate cells (PrEC, PrSM, and PrSt)
Concentration:	500 μM
Incubation Time:	48 hours
Result:	Increased the sub-G0-G1 cell fraction of LNCaP (41.5%), PC-3 (12.1%), and DU145 cells (11.2%). Caused a significant increases in G2 cells , particularly in LNCaP (13%), PC-3 (32%), and DU145 (20%) cells.

RT-PCR^[3]

Cell Line:	Human thyroid epithelial cells (HTori-3)
Concentration:	5 μM
Incubation Time:	24 hours
Result:	Downregulated the CDC6, GADD45A, FAS and ATF3 in irradiated cells.

体内研究
(In Vivo)

L-SelenoMethionine (0.06-12 μg/g diet; p.o. for 3 d) partially or completely prevent the decrease in the serum or plasma levels of total antioxidants in rats exposed to gamma rays, protons or HZE particles^[5].

Clinical Trial

分子量

196.11

性状

Solid

Formula

C₅H₁₁NO₂Se

CAS 号

3211-76-5

中文名称

L-硒代蛋氨酸

结构分类

Ketones, Aldehydes, Acids

来源

Plants
other families

Widespread

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

溶解性数据

In Vitro:

H₂O : 14.29 mg/mL(72.87 mM;Need ultrasonic)

DMSO : 1 mg/mL(5.10 mM;Need ultrasonic)

配制储备液

浓度溶剂体积质量

1 mg

5 mg

10 mg

1 mM	5.0992 mL	25.4959 mL	50.9918 mL
5 mM	1.0198 mL	5.0992 mL	10.1984 mL
10 mM	0.5099 mL	2.5496 mL	5.0992 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.
请依序添加每种溶剂： PBS
Solubility: 9.09 mg/mL (46.35 mM); Clear solution; Need ultrasonic and warming and heat to 60℃

*以上所有助溶剂都可在本网站选购。