Antimicrobial agent-5 是一种强效抗菌剂，对革兰氏阳性菌/阴性菌都具有良好的细胞选择性。Antimicrobial agent-5 能够阻断 LPS 和 CD14/TLR4 受体间的相互作用，表现出抗炎活性并缓解 LPS 诱导的炎症。
| 生物活性 | Antimicrobial agent-5 is an potent antimicrobial agent, and displays excellent cell selectivity against Gram-negative bacteria and Gram-positive bacteria. Antimicrobial agent-5 blocks the interaction between LPS and CD14/TLR4 receptor, and shows anti-inflammatory activity against LPS-induced inflammation[1]. |
体外研究
(In Vitro) | Antimicrobial agent-5 (compound 9) (0.5-32 μg/mL, 16 h; 1-128 μg/mL; 24 h) shows potent biofilm inhibitory (IC50=2 μg/mL) and eradicating activities (IC50=16 μg/mL) against multidrug-resistantPseudomonas aeruginosa(MDRPA)[1].
Antimicrobial agent-5 (5 μgmL, 20 μg/mL; 18 h) inhibits both the release and expression of nitric oxide (NO) and tumor necrosis factor-α (TNF-α) from LPS-stimulated (1 μg/mL) RAW 264.7 cells[1].
Antimicrobial agent-5 exhibits proteolytic resistance and salt/serum stability[1].
Antimicrobial agent-5 (0.5-256 μg/mL; 2 h) exhibits negligible side effects against sheep red blood cells (sRBCs) with hemolytic activity (the minimum hemolytic concentration, MHC) of >256 μg/mL[1].
Cell Viability Assay[1] | Cell Line: | RAW264.7 cells | | Concentration: | 5 μg/mL, 20 μg/mL | | Incubation Time: | 18 hours | | Result: | Decreased TNF-α release at 20 μg/mL, with inhibition rate of 72.44%.
Results reduction in the LPS-stimulated production of NO, with inhibition rate of 31.51%. |
Cell Viability Assay[1] | Cell Line: | E. coli[KCTC 1682],P. aeruginosa[KCTC 1637],S. epidermidis[KCTC 1917],S. aureus[KCTC1621] | | Concentration: | 1-128 μg/mL | | Incubation Time: | 24 hours | | Result: | Inhibited Gram-negative bacteria and Gram-positive bacteria with IC50of 6.1 μM (E. coli[KCTC 1682],P. aeruginosa[KCTC 1637],S. epidermidis[KCTC 1917],S. aureus[KCTC1621]), respectively. |
|
| 分子量 | |
| Formula | |
| 运输条件 | Room temperature in continental US; may vary elsewhere. |
| 储存方式 | Please store the product under the recommended conditions in the Certificate of Analysis. |
