SKF83822 hydrobromide

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本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:

74115-10-9

SKF83822 hydrobromide 是一种多巴胺D1受体激动剂。SKF83822 hydrobromide激活 G_s/_olf/腺苷酸环化酶 (AC) 偶联的 D1 受体，但不激活磷脂酶 C (PLC) 偶联的 D1 样受体。

生物活性

SKF83822 hydrobromide is a potentdopamine D1 receptoragonist. SKF83822 hydrobromide activates G_s/_olf/adenylyl cyclase (AC)-coupled D1 receptors, but notphospholipaseC (PLC)-coupled D1-like receptors^[1].

IC₅₀& Target^[1]

D₁Receptor

体外研究
(In Vitro)

SKF83822 (1 μM) increases DARPP-32 phosphorylation in Neostriatal slices. Treatment with SKF83822 for 5 min stimulates DARPP-32 Thr34 phosphorylation maximally at a concentration of 100 μM with a half maximal effect at ~1 μM. SKF83822 (1 μM) does not affect the phosphorylation of DARPP-32 at Thr75, Ser97 or Ser130^[1].

Western Blot Analysis^[1]

Cell Line:	Neostriatal slices
Concentration:	1 μM
Incubation Time:	0, 0.5, 2, 5, and 10 minutes
Result:	Treatment with 1 μM increased the level of phospho-Thr34 DARPP-32, maximally by ~6-fold within 2 min of incubation.

体内研究
(In Vivo)

SKF83822 activates dopamine D1 receptors coupled to Gαs/olf and downstream cyclase activity. SKF83822 produces a locomotor response in both rodent and non-human primate models without affecting stereotypy, intense grooming, or dyskinesia. An acute injection of SKF83822 (0.4 mg/kg; i.p.) induced a greater than threefold increase in locomotor activity relative to the baseline period for each genotype^[1].

Animal Model:	G_αqknockout mice^[1]
Dosage:	0.4 mg/kg
Administration:
Result:	There was a significant increase in locomotor activity in each genotype.

分子量

424.76

Formula

C₂₀H₂₃BrClNO₂

CAS 号

74115-10-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.