360A 是一种稳定的G-四链体配体，还抑制 TRAP-G4 试验中端粒酶的端粒酶活性，IC50值为 300 nM。

360A is a stabilizing G-Quadruplex ligand, and also inhibits telomerase activity for telomerase in TRAP-G4 assay(IC50 : 300 nM).

A549 lung carcinoma cell lines treatment with 5 μM of 360A led to an inhibition of cell proliferation within 11 days of treatmen. Treatment with the newly synthesized dimer (360A)2A led to a comparable antiproliferative effect[1]

Supplemented with 10% fetal calf serum (FCS) and 1% penicillin/streptomycin. A549 cells were maintained in humidified incubators at 37 °C in an atmosphere containing 5% CO2 and ambient oxygen of 20%. Cells were grown in 25 cm^2 flasks (150,000 cells/flask) and treated with 5 μM of 360A or (360A)2A every 3 or 4 days (i.e., at each reseeding) until cultures were terminated. Control cells were either non-treated or treated with 0.1% DMSO. At each reseeding the remaining cells were pelleted and snap frozen for further DNA extraction.Statistical analysis was performed using GraphPad Prism 5. A Chi-square test was used to compare TDEs frequencies[1].

794458-56-3

C27H23N5O2

449.513

3,3'-[2,6-吡啶二基二(羰基亚氨基)]二[1-甲基喹啉鎓]

DMSO:10 mM
Powder: -20°C for 3 years
In solvent: -80°C for 2 years