| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 询价 |
| 25mg | 询价 |
Leupeptin, Microbial is a broad-spectrum serine and cysteine protease inhibitor.
Preparation Method | The concentration of Leupeptin, Microbial was calculated by enzyme inhibition assay using 50 pg of papain and 0.2 mM of N-benzoylL-arginine ethyl ester as the target protease and papain substrate, respectively. Enzyme reactions were carried out at 25 ℃ and pH 6.2. |
Applications | Leupeptin, Microbial is a leucine-specific protease and a metalloprotease. |
Cell lines | MRC-C cell |
Preparation Method | To investigate the effect of Leupeptin, Microbial on virus yield, MRC-C cultures in 35 mm dishes were infected by adsorption of virus from 0T ml concentrated suspension of HCV 229E for 1 h at 36 ℃. The cultures were washed twice and overlaid with 2 ml Eagle’s MEM plus 0.2% bovine serum albumin and 20 mm-HEPES buffer pH 7.4. Leupeptin, Microbial at a range of concentrations was added to the virus inoculum, to the maintenance medium and also to maintenance medium used to treat the cultures for 1 h before infection. After 24 h at 36 ℃ in air, the cells were scraped into the medium and disrupted by sonication. |
Reaction Conditions | 0.4 to l00 μg/ml for 25 h（1 h before infection to 24 h after infection） |
Applications | The protease inhibitor Leupeptin, Microbial prevented multiplication of the human coronavirus strain 229E in cultures of MRC-C cells. |
Animal models | C57BL/6NCrl male mouse |
Preparation Method | Mice received i.p. injections of 0.5 ml sterile Phosphate Buffered Saline or 0.5 ml PBS containing 9–40 mg/kg Leupeptin, Microbial hemisulfate. |
Dosage form | 0, 9, 18 36 and 40 mg/kg Leupeptin, Microbial; Intraperitoneal injection |
Applications | Rate of LC3b accumulation after Leupeptin, Microbial treatment was greatest in the liver and lowest in spleen. |
| 文献引用 | |
| 产品描述 | Leupeptin, Microbial is a broad-spectrum serine and cysteine protease inhibitor[1]. Leupeptin, Microbial is a reversible protease inhibitor, It acts on bovine trypsin, human plasminase, bovine splenic cathepsin B, and recombinant human caltrypsin with Ki values of 35 nM, 3.4 μM, 6 nM, and 72 nM[7]. The protease inhibitor Leupeptin, Microbial prevented multiplication of the human coronavirus strain 229E in cultures of MRC-C cells[3]. Leupeptin, Microbial-mediated inhibition of trypsin-like proteases maintains substrate mycelium development, whereas proteolytic degradation of Leupeptin, Microbial in stationary phase cultures derepresses the trypsin-like proteases, leading to the digestion of substrate mycelium and promotion of aerial mycelium formation[2]. As the calpain inhibitor Leupeptin, Microbial primarily protected hair cells from neomycin[5]. The expression of hepatitis B surface antigen (HBsAg) from Leupeptin recovered up to 50% of the cell suspension culture[6]. Leupeptin, Microbial was well tolerated in mice. Leupeptin, Microbial significantly increased LC3b-II in a dose-dependent way in the total tissue extract and lysosomal enrichment portion (LE portion). At the level of electron microscopy (EM), Leupeptin, Microbial induced the accumulation of electron-dense vesicle structures[4]. References: |
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