CID2011756 是一种具有抗肿瘤活性的ATP 竞争性广谱PKD抑制剂，对 PKD1 的IC50值为 3.2 μM，在细胞中对 PKD2 和 PKD3 的IC50值分别为 0.6 和 0.7 μM。

CID-2011756 is an ATP-competitive and specific PKD1 inhibitor.

Ub-PLA2 assay: Recombinant enzymes in 20 mM Tris-HCl, pH 8.0, 2 mM CaCl2 and 2 mM β-mercaptoethanol (DUB assay buffer) are preincubated with single doses or dose ranges of PR-619 or P22077 for 30 minutes in a 96 well plate before the addition of Ub-PLA2 and NBD C6-HPC. The liberation of a fluorescent product within the linear range of the assay is monitored at room temperature using a fluorescence plate reader. Vehicle (2%(v/v) DMSO) and 10 mM N-ethylmaleimide are included as controls. Where ≥60% inhibition is observed, EC50 values are determined using a sigmoidal dose response equation.

638156-11-3

C22H21ClN2O3

396.87

CID2011756;CID-2011756

DMSO:19.8 mg/mL(50 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years