Gramine是从芦竹中得到的一种生物碱，是脂联素受体激动剂，对 AdipoR2 和 AdipoR1 的IC50值分别为 3.2 和 4.2 μM。它也是小鼠和人β2-肾上腺素受体激动剂，具有抗肿瘤、抗炎和抗病毒作用。

Gramine, a natural indole alkaloid, present in several plant species, acts as an active adiponectin receptor (AdipoR) agonist, with IC50s of 3.2 and 4.2 μM for AdipoR2 and AdipoR1, respectively. Gramine is also a human and mouse β2-Adrenergic receptor (β2-AR) agonist. Gramine has anti-tumor, anti-viral and anti-inflammatory properties.

Assay of protein kinase C: Protein kinase C is arrayed by measuring 32PI transferred from [gamma-32PI] ATP to lysine-rich histone type Ill-s. The reaction mixture (80 μL) contained 50 mM Tris-HCI. pH 7.4, 100 μM CaCl2, 10 mM MgCI2, 37.5 μL/mL histone type Ill-s, 10 μM [gamma-32PI] ATP (1250 cpm/pmol), 31 μM bovine brain phosphatidylserine and 0.5 μM 1,2 sn-dioleylglycerol. Fifteen μL of purified PKC (final concentration in assay 0.38 μg/mL) is added to the incubation mixture. After 10 min at 30°C, the reaction is stopped by addition of 30 μL of casein 30 mg/mL and 0.9 ml of 12% trichloroacetic acid. The acid precipitable material is collected by centrifugation, dissolved in 1N NaOH (100μL) and precipitated again with 1 ml of 12% trichloroacetic acid. The pellet is dissolved in 1N NaOH (100μL) and 32P incorporation is measured by scintillation counting in Aquasol.

87-52-5

C11H14N2

174.247

芦竹碱;NSC 16892;Donaxine

H2O:<1 mgml
Ethanol:33 mg/mL (189.4 mM)
DMSO:33 mg/mL (189.4 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years