HJC0350 是一种有效地、选择性的EPAC2拮抗剂(IC50:0.3 μM)。
产品描述
HJC0350 is an effective and specific EPAC2 inhibitor (IC50: 0.3 μM), and no inhibition of Epac1.
体外活性
HJC0350 selectively blocks cAMP-induced EPAC2 activation but do not inhibit cAMP-mediated PKA activation. In HEK293/EPAC2-FL cells, HJC0350 also inhibits 007-AM mediated cellular activation of EPAC2. [1]
激酶实验
In Vitro IRE1 RNase and RIDD Assays: Analysis of radiolabeled Xbp1 substrate cleavage is performed as previously except that mammalian IRE1 reaction buffer is used. In vitro RIDD substrates are synthesized by in vitro transcription using the T7-MAXIscript Kit in the presence of 32P ATP or Cy5-UTP on templates isolated by RT-PCR from mouse Min6 cells (Ins2) or PCR from cloned XBP1 cDNA. The resulting products are gel purified to obtain full-length substrate. Reactions are then separated by 15% UREA-PAGE for analysis by phosphorimaging or by near-infrared imaging using the LI-COR Odyssey scanner.
Cas No.
885434-70-8
分子式
C15H19NO2S
分子量
277.38
储存和溶解度
DMSO:13.9 mg/mL (50 mM)
Ethanol:5.6 mg/mL (20 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
