Amarogentin 是要从Swertia和Gentiana根中提取的裂环烯醚萜苷，有抗氧化、抗肿瘤和抗糖尿病活性，具有保肝和免疫调节作用。它通过激活AMPK发挥有益的血管代谢作用。它促进细胞凋亡，阻止 G2/M 细胞周期和下调 PI3K/Akt/mTOR 信号通路。

Amarogentin is mainly extracted from Swertia and Gentiana roots. It plays cemopreventive/therapeutic role during liver carcinogenesis through modulation of cell cycle and apoptosis

Amarogentin, a bitter secoiridoid glycoside from S. chirayita, shows varied activity in several patho-physiological conditions, predominantly in leishmaniasis and carcinogenesis.?Experimental analysis has revealed that Amarogentin downregulates the cyclooxygenase-2 (COX-2) activity and helps to curtail skin carcinogenesis in mouse models;?however, there exists no account on selective inhibition of the inducible cyclooxygenase (COX) isoform by Amarogentin[1].marogentin inhibits the growth of SNU-16 human gastric cancer cells (IC50 = 12.4 μM after 48 hours) and increases apoptosis when used at a concentration of 50 μM. Amarogentin (10-50 mg/kg, s.c.) dose-dependently reduces tumor growth in a SNU-16 nude mouse xenograft model[2].

The computer-aided drug discovery methods were used to unravel the COX-2 inhibitory mechanism of Amarogentin and to check its selectivity for the inducible isoform over the constitutive one.?The generated theoretical models of both isoforms were subjected to molecular docking analysis with Amarogentin and twenty-one other Food and Drug Authority (FDA) approved lead molecules.?The post-docking binding energy profile of Amarogentin was comparable to the binding energy profiles of the FDA approved selective COX-2 inhibitors.?Subsequent molecular dynamics simulation analysis delineated the difference in the stability of both complexes, with Amarogentin-COX-2 complex being more stable after 40ns simulation.?The total binding free energy calculated by MMGBSA for the Amarogentin-COX-2 complex was -52.35 KCal/mol against a binding free energy of -8.57 KCal/mol for Amarogentin-COX-1 complex, suggesting a possible selective inhibition of the COX-2 protein by the natural inhibitor.?Amarogentin achieves this potential selectivity by small, yet significant, structural differences inherent to the binding cavities of the two isoforms.?Hypothetically, it might block the entry of the natural substrates in the hydrophobic binding channel of the COX-2, inhibiting the cyclooxygenation step[1].

21018-84-8

C29H30O13

586.546

苦杏苷;苦龙胆酯苷

DMSO:2 mg/mL (3.4 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years