Hoechst 34580 tetrahydrochloride, a cell-permeable fluorescent dye, is used for staining DNA and nuclei.

Hoechst 34580 is a good candidate for treating Alzheimer's disease by inhibiting Aβ formation. 50 μM Aβ42 solutions co-incubated with 100, 25, 12.5, 3.125, 0.78, and 0.1, 0.01 μM Hoechst 34580 at 37 °C for 70 h. Hoechst 34580 can inhibit the aggregation of Aβ42 in a dose-dependent manner. The IC50 is obtained by measuring the concentration of Hoechst 34580 while maintaining the Aβ42 concentration which gave 0.86±0.05 μM for Hoechst 34580.

T19013

C27H33Cl4N7

597.41

Hoechst 34580 tetrahydrochloride (23555-00-2 free base);HOE 34580 tetrahydrochloride

H2O:41.2 mg/mL (68.96 mM),Need ultrasonic and warming
Powder: -20°C for 3 years
In solvent: -80°C for 2 years