Amphotericin B是一种抗真菌剂，对许多真菌物种具有广谱活性。

Amphotericin B is an antifungal agent with a broad spectrum of activity against many fungal species.

在仓鼠瘙痒病模型中,Amphotericin B能够降低PrPSc的积累.在传染性亚急性海绵状脑病（TSSE）小鼠中,Amphotericin B能够显著降低PrPSc水平.

在表达TLR2 and CD14的细胞中,Amphotericin B诱导信号转导和炎性细胞因子的释放。当形成小阳离子和阴离子可渗透的水性孔隙时,两性霉素B仅杀死单细胞利什曼原虫鞭毛体（LP）。

THP-1 and HEK293 cells are transiently transfected using DEAE-dextran and Polyfect reagent, respectively. Plasmids transfected contain genes coding for the NF-κB-dependent pELAM-luc luciferase reporter, TLR2, TLR4, CD14, and MD2. Cells (5×105 THP-1 or 1×105 HEK293) are added to 12-well plates, washed after 18 h, and stimulated for 5 h. Cells are then lysed with reporter lysis buffer as directed, and lysates are analyzed for luminescence using Promega luciferase substrate and a Monolight 3010 luminometer.

Amphotericin B is dissolved in DMSO. The kinetics of cell death induced by AmB against Leishmania promastigotes is followed by using fluorometry with the DNA-binding compound ethidium bromide (EB). Fluorescence measurements are performed on a SPEX Fluorolog II spectrophotometer at 365-580 nm excitation-emission wavelengths. Promastigotes at a final concentration of 25×106 cells/mL are incubated for 5 min with gentle stirring in the fluorescence cuvette with 2 mL of different buffered solutions but always containing 10 mM glucose and EB (50 mM). After signal stabilization is achieved, AmB is added and dissolved in dimethylsulfoxide. Maximal EB incorporation is always obtained by adding digitonin (50 mg/mL). All solutions used are buffered with 75 mM TRIS (pH 4 7.6) and contain 150 mM NaCl (BNa+), 150 mM KCl (BK+), 150 mM choline chloride, and 100 mM sucrose, 100 mM NaCl. The osmolarity of all solutions is always adjusted to 390±5 mOsm using an advanced instrument SW2 osmometer.

1397-89-3

C47H73NO17

924.091

两性霉素B;NSC 527017

DMSO:21 mg/mL (22.7 mM)
Ethanol:<1 mgml
H2O:<1 mgml
Powder: -20°C for 3 years
In solvent: -80°C for 2 years