Hoechst 33258 analog 3 是一种Hoechst染料，可与DNA结合，发蓝色荧光。

Hoechst 33258 analog 3 is part of a family of blue fluorescent dyes used to stain DNA.

The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similar excitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around an emission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethylformamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage, the solutions are instead frozen at ≤-20 °C. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells.

23554-98-5

C26H26N6

422.52

DMSO:3.57 mg/mL (8.45 mM),Need ultrasonic and warming and heat to 70°C
Powder: -20°C for 3 years
In solvent: -80°C for 2 years