DX600 是一种angiotensin-converting enzyme 2 (ACE2)的特异抑制剂，不会与ACE发生交叉反应。

DX600 is an ACE2 specific inhibitor that does not cross-react with ACE. Three human cell lines (Calu-3, Caco-2, Huh-7) are evaluated for the cell surface expression of ACE2 by flow cytometry and Western blot. Subsequently, whole cell lysates, cell culture supernatant, and live cells are evaluated in the assay. Calu-3 has elevated levels of ACE2 compared to Caco-2 or Huh-7. Calu-3 also demonstrates elevated ACE2 enzymatic activity in all three sources and can be inhibited by DX600.[1]

Mouse ACE2-driven Ang-(1-7) formation from Ang II is blocked by MLN-4760 but not by either linear or cyclic DX600. DX600 only blocks human ACE2 activity.[2]

Cell lines: Three human cell lines (Calu-3, Caco-2, Huh-7) Concentrations: 10 μM Incubation Time: 1 h Method: To measure NAb activity against endogenous ACE2, the recombinant ACE2 is replaced by cellular ACE2. 100 μL of cell lysate at 4 mg/mL or same volume of cell culture supernatant is pre-incubated with either PCs at a final concentration of 10 μg/mL, equal volume control serum, DX600, ACE2 specific inhibitor at final concentration of 10 μM, or equal volume of control buffer (PBS) at room temperature for 1 h.

478188-26-0

C141H185N35O40S2

3074.32

DX600

(< 1 mg/ml refers to the product slightly soluble or insoluble )
Powder: -20°C for 3 years
In solvent: -80°C for 2 years