Sulfo-NHS-SS-Biotin sodium 是一种长链可裂解和细胞无渗透的胺类活性生物素化 (biotinylation) 试剂。Sulfo-NHS-SS-Biotin sodium 可用于细胞表面蛋白 (cell-surface protein) 的标记和纯化。

Sulfo-NHS-SS-Biotin sodium is a cell-impermeant and long-chain cleavable amine-reactive biotinylation reagent. It can be used for the labeling and purifying of cell-surface protein.

Sulfo-NHS-SS-biotin is a negatively charged reagent and does not permeate cell membranes. Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides[1]. Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail [2].

325143-98-4

C19H28N4NaO9S4

607.7

Sulfo-NHS-SS-Biotin;Sulfosuccinimidyl 3-[[2-(biotinamido)ethyl]dithio]propionate;Sulfo-NThiol-SS-biotin;Sulfo-NHS-SS-Biotin (sodium);Sulfo-NHS-SS-Biotin sodium

DMSO:100 mg/mL (164.8 mM),Need ultrasonic
H2O:180 mg/mL (296.7 mM),Need ultrasonic
Powder: -20°C for 3 years
In solvent: -80°C for 2 years