生物活性
Nifedipine (BAY-a-1040), a potent vasodilator agent with calcium antagonistic action. It is a useful anti-anginal agent that also lowers blood pressure. Nifedipine (BAY-a-1040) is a dihydropyridine calcium channel blocker. Its main uses are as an antianginal (especially in Prinzmetal’s angina) and antihypertensive, although a large number of other uses have recently been found for this agent, such as Raynaud’s phenomenon, premature labor, and painful spasms of the esophagus in cancer and tetanus patients. It is also commonly used for the small subset of pulmonary hypertension patients whose symptoms respond to calcium channel blockers.
化学数据
| 分子量 | 346.33 |
| 分子式 | C17H18N2O6 |
| CAS号 | 21829-25-4 |
| 纯度 | >99% |
| 溶解性（25°C） | DMSO 59 mg/mL |
| 储存和运输条件 | -20°C, protect from light, sealed
常温运输及临时存放 |
实验操作 来自于公开的文献，仅供相同实验参考（如实验材料、目的不同，请参考其他文献）
| 细胞实验 |
|---|
| 细胞系 | TM4 cells |
| 方法 | Effects of extracellular calcium ion and calcium channel blocker on Sertoli cell phagocytosis. TM4 cells were cultured for two hours and then treated with or without 10 μM nifedipine for additional 20 hours. Fluorescent carboxyl microspheres were then added to wells for further two hours as usual. Culture medium of some untreated wells was replaced by one mM EGTA containing culture medium (Ca; 0.1 mM) just before addition of microspheres. Cells were then evaluated for phagocytosis by flow cytometric analysis. Calcium concentration of untreated or one mM EGTA growth medium was one mM or 0.1 mM, respectively. |
| 浓度 | 10 μM |
| 处理时间 | 20 hours |
不同实验动物依据体表面积的等效剂量转换表（数据来源于FDA指南）
| 小鼠 | 大鼠 | 兔 | 豚鼠 | 仓鼠 | 狗 |
| 重量 (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| 体表面积 (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km系数 | 3 | 6 | 12 | 8 | 5 | 20 |
| 动物 A (mg/kg) = 动物 B (mg/kg) × | 动物 B的Km系数 |
| 动物 A的Km系数 |
例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的Km系数（3），再除以大鼠的Km系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。
储备液配制
以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 2.8874 mL | 14.4371 mL | 28.8742 mL |
| 5 mM | 0.5775 mL | 2.8874 mL | 5.7748 mL |
| 10 mM | 0.2887 mL | 1.4437 mL | 2.8874 mL |
