MTT

立即咨询

MTT

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

CAS NO:	298-93-1
规格:	≥98%

包装与价格：

包装	价格(元)
1g	询价
2g	询价
5g	询价
10g	询价

理化性质和储存条件

	Molecular Weight: 414.32 Formula: C18H16BrN5S CAS No.: 298-93-1 SMILES: CC1=C(C)N=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=CC=C4)S1.[Br-]
Storage	-20℃ for 3 years in powder form
Storage	-80℃ for 2 years in solvent
Technical Information	Thiazolyl Blue; Thiazolyl Blue Tetrazolium Bromide; Methylthiazolyldiphenyl-tetrazolium bromide

实验参考方法

In Vitro	MTT is used in conjunction with rhodamine B to measure mitochondrial membrane potential. MTT-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of MTT is strong. MTT is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].
References	[1]. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.

In Vitro

MTT is used in conjunction with rhodamine B to measure mitochondrial membrane potential. MTT-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of MTT is strong. MTT is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].

References

[1]. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.