| CAS NO: | 637-01-4 |
TMPD dihydrochloride是一种用于研究细胞色素c的膜栓系化合物，用于研究其对酵母细胞死亡的影响。TMPD dihydrochloride 也是一种 complex IV 的底物。在氧化酶试验中，通常使用氧化还原介质N、N、N '、N'-四甲基对苯二胺 (TMPD)进行比色鉴定细胞培养中的病原菌和非病原菌。使用TMPD在COX活性的高通量微孔板测定中可能成为筛选抑制体内 COX 活性的潜在治疗剂的首选方法。
| 分子量 | 237.17 |
| 分子式 | C10H18Cl2N2 |
| CAS号 | 637-01-4 |
| 纯度 | >98% |
| 溶解性（25°C） | Water ≥ 60 mg/mL DMSO ≥ 10 mg/mL |
| 储存和运输条件 | 4°C, protect from light, dry, sealed 常温运输及临时存放 |
| 细胞实验 | |
|---|---|
| 细胞系 | Escherichia coli & Bacillus subtilis |
| 方法 | Bacteria culture and oxidase test Escherichia coli Bacteria were cultured in 2 × TY liquid microbial growth medium (broth), containing 16 g/L tryptone, 10 g/L yeast extract and 5.0 g/L NaCl. Growth medium was inoculated with bacteria from frozen stocks and incubated in glass culture flasks for 18 h at 37 °C in an incubator shaker. An E. coli suspension of 50 μL was transferred into a new culture flask, containing fresh growth medium. Following incubation for 3 to 4 h at 37 °C, the number of bacteria in solution was determined by optical density (OD) at a wavelength of 600 nm (OD600 of 1.0 = 8 × 108cells/mL). When an OD600 between 0.4 and 1.8 was reached, bacteria were harvested by centrifugation for 15 min at 3000 rcf and re-suspended in pre-warmed (37 °C) PBS. Bacillus subtilis (strain PY79) Low salt growth medium (broth), containing 10 g/L tryptone, 5.0 g/L yeast extract and 5.0 g/L sodium chloride, was inoculated with cultures from frozen stocks and incubated at 30 °C for 36 h in an incubator shaker. An OD600 was determined to calculate number of bacteria in solution (OD600 of 1.0 = 5 × 108cells/mL). Bacteria were harvested by centrifugation for 10 min at 300 rcf and re-suspended in pre-warmed (30 °C) PBS. Cultures grown on agar, containing 10 g/L tryptone, 5.0 g/L yeast extract 5.0 g/L sodium chloride and 20 g/L agar, were inoculated onto agar plates and grown at 30 °C for 36 h in a static incubator. For the oxidase test, 200 μL of a 1% (wt) N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD-2HCl) solution was added to 1 mL of cell suspension (1 × 107cells/μL). Images were taken within 30 s of solution mixture. |
| 浓度 | 200 μL of a 1% (wt) N,N,N′,N′-tetramethyl-p-phenylenediamine dihydrochloride (TMPD-2HCl) solution |
| 处理时间 | - |
| 动物实验 | |
|---|---|
| 动物模型 | |
| 配制 | |
| 剂量 | |
| 给药处理 | |
| 小鼠 | 大鼠 | 兔 | 豚鼠 | 仓鼠 | 狗 | |
| 重量 (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| 体表面积 (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km系数 | 3 | 6 | 12 | 8 | 5 | 20 |
| 动物 A (mg/kg) = 动物 B (mg/kg) × | 动物 B的Km系数 |
| 动物 A的Km系数 |
例如，依据体表面积折算法，将化合物用于小鼠的剂量20 mg/kg 换算成大鼠的剂量，需要将20 mg/kg 乘以小鼠的Km系数（3），再除以大鼠的Km系数（6），得到化合物用于大鼠的等效剂量为10 mg/kg。
以下数据基于产品分子量，对于特殊产品，请参照COA中的储备液配制条件和说明进行操作。
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.2164 mL | 21.0819 mL | 42.1638 mL |
| 5 mM | 0.8433 mL | 4.2164 mL | 8.4328 mL |
| 10 mM | 0.4216 mL | 2.1082 mL | 4.2164 mL |
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