Used for detection of β-lactamases
CAS：41906-86-9
分子式：C21H16N4O8S2
分子量：516.5
纯度：98%
存储：Store at -20°C

Background:

Nitrocefin?is a?chromogenic?cephalosporin?substrate which is used for detection of β-lactamases. β-lactamases are enzymes produced by various microbes that mediate resistance to?β-lactam?antibiotics such as?penicillins and ?cephamycins. Comparing to other methods for β-lactamases detection, nitrocefin is more sensitive while using few materials and inexpensive equipment. The color change by nitrocefin can be followed quantitatively by measuring changes in absorption between 380 to 500 nm region[1].

Reference:
[1] O'Callaghan, Cynthia H et al.? Novel Method for Detection of B-Lactamases by Using a Chromogenic Cephalosporin Substrate. Antimicrobial Agents and Chemotherapy. 1972 Apr; 1(4):283-288.