DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO).
CAS：205391-01-1
分子式：C20H14N2O5
分子量：362.3
纯度：98%
存储：Store at -20°C

Background:

DAF-2 is a sensitive fluorescent indicator commonly used for the detection of nitric oxide (NO).[1],[2] It reacts with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nM, respectively.2 At neutral pH the detection limit for NO is 2-5 nM.

Reference:

[1]. Kojima, H., Sakurai, K., Kikuchi, K., et al. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chemical & Pharmaceutical Bulletin 46, 373-375 (1998).
[2]. Nakatsubo, N., Kojima, H., Kikuchi, K., et al. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: Diaminofluoresceins. FEBS Letters 427, 263-266 (1998).