L-Buthionine-(S,R)-sulfoximine是一种具有细胞渗透性的、有效的、快速起效的、不可逆的G-谷氨酸半胱氨酸合成酶(γ-glutamylcysteinesynthetase)抑制剂，可降低细胞内谷胱甘肽的水平，其对黑色素瘤、乳腺卵巢癌标本的IC50值分别为1.9μM、8.6μM和29μM。
CAS：83730-53-4
分子式：C8H18N2O3S
分子量：222.31
纯度：98%
存储：Store at -20°C

Background:

L-Buthionine-(S,R)-sulfoximine is a cell-permeable, potent, fast acting and irreversible inhibitor of g-glutamylcysteine synthetase and depletes cellular glutathione levels. The IC50 value of L-Buthionine-(S,R)-sulfoximine on melanoma, breast and ovarian tumor specimens are 1.9 μM, 8.6 μM, and 29 μM, respectively.

L-Buthionine-(S,R)-sulfoximine (BSO: 50 μM) treatment for 48 hr results in a 95% decrease in ZAZ and M14 melanoma cell line GSH levels, and a 60% decrease in GST enzyme activity. GST-π protein and mRNA levels are significantly reduced in both cell lines[1]. L-Buthionine-(S,R)-sulfoximine (BSO) induces oxidative stress in a cell by irreversibly inhibiting g-glutamylcysteine synthetase, an essential enzyme for the synthesis of glutathione (GSH)[2].

The average number of eye-spots (mean±SEM) is 5.36±0.29 (n=46), 7.79±0.45 (n=34) and 8.78±0.61 (n=32) in untreated controls, 2 mM L-Buthionine-(S,R)-sulfoximine (BSO) and 20 mM BSO treated mice, respectively. The 2 mM BSO treatment results in