Ac-WEHD-AFC 是一种荧光 caspase-1 底物。Ac-WEHD-AFC 可检测 caspase-1 荧光活性，用于肿瘤和炎症的研究。

CAS：210344-99-3
分子式：C38H37F3N8O11
分子量：838.74
纯度：98%
存储：Store at -20°C

Background:

In vitro:Caspase-1 Fluorogenic Activity Assay:^[1]

1.Incubate the cells according to your normal protocol. 

2. Cells are transiently transfected with caspase-1 in combination with pcDNA3.1, Myc-cIAP2, Myc-cIAP1, or Myc-XIAP and collected 24 hr later. 

3. Cells were lysed in CHEGG buffer before sonicating for 15 s at 60% amplitude (for cleavage assays). 

4. Lysates were incubated with 10 mM of the fluorogenic caspase-1 substrate Ac-WEHD-AFC. 

5. The release of free AFC was monitored continuously for 1 hr (excitation 380 nm, emission 460 nm) in 1 min intervals and expressed as arbitrary fluorescence units per minute.

参考文献：

[1]. Katherine Labbé, et al. Cellular inhibitors of apoptosis proteins cIAP1 and cIAP2 are required for efficient caspase-1 activation by the inflammasome. Immunity