rGHRH(1-29)NH2

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rGHRH(1-29)NH2

本产品不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

规格:	98%
分子量:	3473.02

包装与价格：

包装	价格(元)
1mg	询价
5mg	询价
10mg	询价
25mg	询价

rGHRH(1-29)NH2是能刺激生长激素(GH)分泌的合成肽。
货号:ajcx13238
CAS:N/A
分子式:C155H251N49O40S
分子量：3473.02
溶解度:H2O : ≥ 50 mg/mL (14.40 mM)
纯度：98%
存储：Store at -20°C
库存：现货

Background:

rGHRH(1-29)NH2 is a synthetic peptide which can stimulate the growth hormone (GH) secretion.

Time course studies of rGRF(1-29)NH2 disappearance show apparent half-lives of 18±4 min and 13±3 min in serum and liver homogenate, respectively. This is accompanied by the appearance of degradation products that are all less hydrophobic than the native peptide. In the serum, two major metabolites are detected and isolated by preparative HPLC[1].

[1]. Boulanger L, et al. Catabolism of rat growth hormone-releasing factor(1-29) amide in rat serum and liver. Peptides. 1992 Jul-Aug;13(4):681-9.

Protocol:

Cell experiment:

To isolate GRF metabolites in the liver, rGRF(1-29)NH2 (10 mg) is first preincubated in 232 mL of Krebs' buffer (5 min, 37°C) to help GRF solubilization and then incubated with a liver homogenate in a shaking bath at 37°C. The homogenate is prepared as in the degradation assays with 580 mg of liver (10 mg/mL). The reaction is stopped after 30 min by adding 174 mL of cold 50 mM phosphate solution (pH 0.8) and centrifugation (48,000×g, 20 min, 4°C). The supernatant is filtered twice and its pH is adjusted (3.0) with 6 N NaOH before chromatography. The GRF metabolites and residual rGRF(1-29)NH2 are isolated[1].

参考文献:

[1]. Boulanger L, et al. Catabolism of rat growth hormone-releasing factor(1-29) amide in rat serum and liver. Peptides. 1992 Jul-Aug;13(4):681-9.