WL47TFA是一种高亲和力的cavolin-1(CAV1)ligand(Kd=23nM)，是一种有效的CAV1寡聚体破坏剂。WL47TFA对CAV1的选择性高于BSA、酪蛋白和HEWL。WL47TFA比原始T20亲本序列长度小80%，可用于caveolin-1功能的研究。
货号:ajcx31838
CAS:N/A
分子式:C82H131N24F3O20S4
分子量：1958.32
溶解度:N/A
纯度：98%
存储：Store at -20°C
库存：现货

Background:

WL47 TFA, a high-affinity cavolin-1 (CAV1) ligand (Kd=23 nM), is a potent disrupter of CAV1 oligomers. WL47 TFA shows selectivity for CAV1 over BSA, casein and HEWL. WL47 TFA is 80% smaller in length than the original T20 parent sequence and can be used for the study of caveolin-1 function[1].

Caveolin-1 (CAV) is a monotonic membrane protein, 22 kDa, it penetrates only one leaflet of the lipid bilayer, and both the N- and C-termini remain on the cytoplasmic side. Multiple copies of CAV oligomerize can form high molecular weight complexes that bend the membrane inward to form invaginations, termed "caveolae," of 50-100 nm in diameter.T20 is a 36 amino acid peptide derived from gp41 and blocks HIV viral fusion with CD4+ T-cells. WL47 is 80% smaller in length and has 7500-fold greater affinity than the original T20 parent sequence.In vitro, Demonstrating WL47 activity with CAV oligomers and a method for measuring the degree of oligomerization. A variant of CAV (CAV(FLV)) that spontaneously oligomerizes to form CAV nanoparticles with diameters is used to examine deoligomerization by WL47. WL47 effectively disrupts these nanoparticles, but it does not disrupt oligomerization in the presence of a reducing agent, this demonstating that WL47 function requires dimerization by disulfide bond

[1]. Amanda J H Gilliam,et al. Affinity-Guided Design of Caveolin-1 Ligands for Deoligomerization. J Med Chem. 2016 Apr 28;59(8):4019-25.