N-乙基马来酰亚胺（NEM）来源于马来酸，它可以烷基化游离的巯基。
货号:ajcx13604
CAS:128-53-0
分子式:C6H7NO2
分子量：125.13
溶解度:DMSO : 50 mg/mL (399.58 mM) H2O : 50 mg/mL (399.58 mM), Ethanol : 12.5 mg/mL (99.90 mM)
纯度：98%
存储：Store at -20°C
库存：现货

Background:

N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[1]. N-ethylmaleimide specific inhibits phosphate transport in mitochondria[2]. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor[3].

参考文献:
[1]. Wu KH, et al. Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolyticactivity of CvaB, an ATP-binding cassette transporter. J Biol Chem. 2004 Jan 9;279(2):901-9.
[2]. Hatase O , et al. Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide. Journal of Bioenergetics, 1973, 5(1):1-15.
[3]. Choo YS, et al. Detection of protein ubiquitination. J Vis Exp. 2009;(30):1293. Published 2009 Aug 19. doi:10.3791/1293