RT-AM是realthiol前体药物。RealThiol是一种基于可逆反应的荧光探针，可以定量监测活细胞中谷胱甘肽的实时动态变化。
货号:ajcx13566
CAS:N/A
分子式:C26H25N3O11
分子量：555.49
溶解度:Soluble in DMSO
纯度：98%
存储：Store at -20°C
库存：现货

Background:

RT-AM is a pro-drug real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.

To enhance the cell permeability of Real Thiol, the carboxylic acid groups is converted to acetoxymethyl (AM) esters, which are readily hydrolysed by esterases to regenerate Real Thiol inside cells. For cells treated with the RT-AM, real-time ratiometric images of the rapid changes of intracellular GSH concentrations in single cells can be generated by dividing the fluorescence intensity values for the 405 nm channel by the 488 nm channel at each corresponding pixel[1].

[1]. Jiang X, et al. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087.

Protocol:

Cell experiment:

The HeLa cell line is grown in DMEM media supplemented with 10% FBS and 1% 1003 Pen Strep. Cells are cultured under a controlled atmosphere (37°C, 5% CO2). Glass bottom dishes are used for cell culture due to confocal scanning requirements. Cells are treated with RT-AM (1?μM with 1% DMSO in DMEM) for 10-15?min before imaging. Confocal images are acquired with 405?nm laser/418–495?nm filter and 488?nm laser/499–615?nm filter. All the microscope settings are kept consistent in each[1].

参考文献: [1]. Jiang X, et al. Quantitative real-time imaging of glutathione. Nat Commun. 2017 Jul 13;8:16087.