DL-threo-2-methylisocitrate 是异柠檬酸裂解酶 1(ICL1) 的底物。
货号:ajcx11396
CAS:N/A
分子式:C14H14Na6O14
分子量：544.19
溶解度:Soluble in DMSO
纯度：98%
存储：Store at -20°C
库存：现货

Background:

DL-threo-2-methylisocitrate sodium is a substrate of isocitratelyase 1(ICL1).

ICL1 had dual activity for both threo-D(s)L(s)-isocitrate (ICA) and DL-threo-2-methylisocitrate (MICA). Using Michaelis-Menten non-linear least squares fit, the Km of purified recombinant ICL1 for threo-D(s)L(s)-isocitrate (ICA) was determined to be 188 mM with akcatof 5.24 s-1 .The Km of ICL2 for MICA was greater than 15 mM which was too high to be determained accurately due to the limitations of the solubility of MICA. The Kcat/Km for ICL1, a measureforcatalyticefficiency, was 2.79 × 104 M-1 s-1 and 1.74 ×103 M-1 s-1, for ICA and MICA respectively.

参考文献:
[1].? Gould TA1,van de Langemheen H,Muoz-Elías EJ,McKinney JD,Sacchettini JC. Dual role of isocitratelyase 1 in the glyoxylate and methylcitrate cycles in Mycobacterium tuberculosis.Mol Microbiol.2006 Aug;61(4):940-7.