A cellimpermeable, negatively charged tetrazolium dye
货号:ajcx23748
CAS:413585-64-5
分子式:C22H16N7O13S2.Na [XH2O]
分子量：650.5
溶解度:DMF: 0.5 mg/ml,DMSO: 3.3 mg/ml,PBS (pH 7.2): 3.3 mg/ml
纯度：98%
存储：Store at -20°C
库存：现货

Background:

XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator.^1,2It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.³

1.Berridge, M.V., Tan, A.S., and Herst, P.M.Tetrazolium dyes as tools in cell biology: New insights into their cellular reductionBiotechnol. Ann. Rev.11127-152(2005) 2.Berridge, M.V., and Tan, A.S.Trans-plasma membrane electron transport: A cellular assay for NADH-and NADPH-oxidase based on extracellular, superoxide-mediated reduction of the sulfonated tetazolium salt WST-1Protoplasma20574-82(1998) 3.Sutherland, M.W., and Learmonth, B.A.The tetrazolium dyes MTS and XTT provide new quantitative assays for superoxide and superoxide dismutaseFree Radical Research27(3)283-289(1997)