刀豆球蛋白 A 是一种 Ca2+/Mn2+- 依赖和甘露糖/葡萄糖结合的植物凝集素，可以在长豆中找到。
货号:ajcx11536
CAS:11028-71-0
分子式:N/A
分子量：N/A
溶解度:Soluble in water
纯度：98%
存储：Store at -20°C
库存：现货

Background:

Concanamycin A belongs to the concanamycins, a family of macrolide antibiotics isolated from Streptomyces diastatochromogenes that are highly active and selective inhibitors of the vacuolar proton-ATPase (v-[H^[+]]ATPase). Among them, concanamycin A is the most selective and potent inhibitor of the V-[H^[+]]ATPase.^[1]

n vitro study indicated that concanamycin A was active at 5 nM and completely blocked influenza virus infection at 10 nM in MDCK cells. Results showed that concanamycin A blocked viral replication by inhibiting the v-[H^[+]]ATPase, thus preventing acidification of endosomes and release of virions into the cytoplasm. An early event in virus infection is the target of concanamytin A. In addition, the inhibition of the v-[H^[+]]ATPase by concanamycin A prevents endosomal acidification, inhibiting virus release from endosomes.^[1]

参考文献:
[1].Rosario G. et al. Concanamycin A blocks influenza virus entry into cells under acidic conditions. FEBS Letters 1994 June; 349; 327-330.

Protocol:

Cell experiment [1]:
Cell lines	CD8+ CTL
Preparation Method	Cells were cultured in the RPMI 1640 medium supplemented with 10% (v/v) FCS and 5% (v/v) culture supernatant of rat spleen cells.
Reaction Conditions	Cells were stimulated with 5 μg/ml of concanavalin A for 24 h.
Applications	Concanavalin A treatment could preferentially decrease the cell viability of CD8+ population prepared from the immunized mice. A certain group(s) of CD8+ population in the immunized mice might be highly susceptible to concanavalin A. The activation through T cell receptors, especially PKC activation, increases the sensitivity of CD8++ CTL to concanavalin A.
参考文献: [1]. Kenichi T. et al. Concanamycin A, a vacuolar type H+-ATPase inhibitor, induces cell death in activated CD8+ CTL. Cytotechnology 1997 July; 25: 127–135.