7-Benzyloxy-4-(trifluoromethyl)coumarin (BFC) is a fluorogenic substrate for the cytochrome P450 (CYP) isoforms CYP1A2 and CYP3A.^1,2Upon enzymatic cleavage by CYP1A2 or CYP3A, 7-hydroxy-4-(trifluoromethyl)coumarin (HFC) is released and its fluorescence can be used to quantify CYP1A2 and CYP3A activity. HFC displays excitation/emission maxima of 410/510 nm, respectively.

1.Renwick, A.B., Surry, D., Price, R.J., et al.Metabolism of 7-benzyloxy-4-trifluoromethyl-coumarin by human hepatic cytochrome P450 isoformsXenobiotica30(10)955-969(2000) 2.Ducharme, J., Sevrioukova, I.F., Thibodeaux, C.J., et al.Structural dynamics of cytochrome P450 3A4 in the presence of substrates and cytochrome P450 reductaseBiochemistry60(28)2259-2271(2021)