7-Ethoxy-4-(trifluoromethyl)coumarin 是细胞色素 P450 超家族各种酶的荧光底物
| Cas No. | 115453-82-2 |
| 别名 | 7-乙氧基-4-(三氟甲基)香豆素 |
| Canonical SMILES | O=C1OC2=C(C(C(F)(F)F)=C1)C=CC(OCC)=C2 |
| 分子式 | C12H9F3O3 |
| 分子量 | 258.2 |
| 溶解度 | Chloroform: slightly soluble,Methanol: slightly soluble |
| 储存条件 | -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request |
| 产品描述 | 7-Ethoxy-5-(trifluoromethyl)coumarin is a fluorogenic substrate for cytochrome P450s (CYPs).1Upon O-deethylation by CYPs, 7-hydroxy-4-(trifluoromethyl)coumarin (HFC) is released and its fluorescence can be used to quantify CYP activity. HFC displays excitation/emission maxima of 410/510 nm, respectively. 1.Buters, J.T.M., Schiller, C.D., and Chou, R.C.A highly sensitive tool for the assay of cytochrome P450 enzyme activity in rat, dog and man. Direct fluorescence monitoring of the deethylation of 7-ethoxy-4-trifluoromethylcoumarinBiochem. Pharmacol.46(9)1577-1584(1993) |
