20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) 是平滑化癌蛋白 (Smo) 的变构激活剂，可在使用 NIH3T3 细胞的基因转录报告基因分析中激活hedgehog (Hh) 信号通路，EC50 为 3 μM。
| Cas No. | 516-72-3 |
| 别名 | 20Α-羟基胆固醇,20α-hydroxy Cholesterol |
| 化学名 | (3S,8S,9S,10R,13S,14S,17S)-17-((S)-2-hydroxy-6-methylheptan-2-yl)-10,13-dimethyl-2,3,4,7,8,9,10,11,12,13,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-3-ol |
| Canonical SMILES | O[C@](CCCC(C)C)(C)[C@@H]1[C@](CC2)(C)[C@@H](CC1)[C@H]3[C@H]2[C@](CC[C@@H]4O)(C)C(C4)=CC3 |
| 分子式 | C27H46O2 |
| 分子量 | 402.65 |
| 溶解度 | ≤20mg/ml in ethanol; 0.1mg/ml in DMSO; 2mg/ml in dimethyl formamide |
| 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request |
| 产品描述 | 20(S)-hydroxyCholesterol (20α-Hydroxycholesterol) is an allosteric activator of the oncoprotein smoothened (Smo) that activates the hedgehog (Hh) signaling pathway with an EC50 of 3 μM in a gene transcription reporter assay using NIH3T3 cells[1][2]. References:
[1]. Nachtergaele S, et al. Oxysterols are allosteric activators of the oncoprotein Smoothened. Nat Chem Biol. 2012 Jan 8;8(2):211-20.
[2]. Nedelcu D, et al, Oxysterol binding to the extracellular domain of Smoothened in Hedgehog signaling. Nat Chem Biol. 2013 Sep;9(9):557-64. |
