| 包装: | 5mg |
| 市场价: | 1800元 |
Cell lines | A549 cells |
Preparation Method | Different experimental groups were added with different concentrations of monensin medium, and the concentrations were set as 1umol/L and 2 jmol/L. |
Reaction Conditions | 1 uM and 2uM monensin for 24h and 48h |
Applications | Monensin could significantly inhibit the proliferation of A549 cells in vitro.Monensin induced G phase arrest in A549 cells, and the proteins related to G phase arrest were changed.Monensin can promote autophagy in A549 cells.Monensin can induce apoptosis in A549 cells. |
Animal models | Male Wistar rats |
Preparation Method | Male Wistar rats were administered monensin at the dose levels of 2.5, 5, and 10 mg/kg. Animals were sacrificed after 67 days of the treatment. The activities of lactate dehydrogenase (LDH), ATPase, acid phosphatase and thiamine pyrophosphatase (TPPase) were measured in the testis. |
Dosage form | 2.5, 5, and 10 mg/kg monensin for 67 days |
Applications | The findings from electron microscopy such as membrane disruption, swelling and disintegration of Golgi apparatus strongly suggest the interference of monensin with the functioning of Golgi apparatus in the spermatogenic cells. Data from the sperm number and motility as well as the fertility studies and the resulted litter size further points towards the antifertility effects of monensin in male rats. |
| 产品描述 | Monensin(protein transport inhibitor) is an ionophore, which can destroy the Golgi apparatus and inhibit intracellular protein transport. Monensin is commonly used as a protein transport inhibitor. Monensin is also often used to inhibit the secretion of secreted proteins such as cytokines, and to enhance the staining of secreted proteins by inhibiting secretion during immunostaining. As an ionophore, Monensin can selectively bind monovalent cations into Li+,Na+,K+,Rb+,Ag+ and Ti+, and transport these cations to the cell membrane[3,4]. Monensin could significantly inhibit the proliferation of A549 cells in vitro.Monensin induced G phase arrest in A549 cells, and the proteins related to G phase arrest were changed.Monensin can promote autophagy in A549 cells.Monensin can induce apoptosis in A549 cells[1]. Monensin is a potent inducer of oxidative stress and inhibitor of androgen signaling leading to apoptosis in prostate cancer cells. Monensin as a potential well-tolerated, in vivo compatible drug with strong proapoptotic effects in prostate cancer cells, and synergistic effects with antiandrogens[5].Nanomolar concentrations of Monensin inhibit early stages of capillary network formation in glioblastoma endothelial cells Monensin inhibits multiple endothelial events, including migration, growth and survival, without affecting adhesion to Matrigel by inhibiting VEGFR and EGFR Mediated signaling pathways act on endothelial cells[2]. Monensin can inhibit the proliferation and migration and activate apoptosis of breast cancer cells via downregulating the expression of UBA2[7]. The findings from electron microscopy such as membrane disruption, swelling and disintegration of Golgi apparatus strongly suggest the interference of monensin with the functioning of Golgi apparatus in the spermatogenic cells. Data from the sperm number and motility as well as the fertility studies and the resulted litter size further points towards the antifertility effects of monensin in male rats[6]. References: |
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