Ac-VDVAD-AFC 是一种半胱天冬酶特异性荧光底物。 Ac-VDVAD-AFC可测定caspase-3样活性和caspase-2活性，可用于肿瘤和癌症的研究。
| Cas No. | 210344-94-8 |
| 别名 | N-Acetyl-Val-Asp-Val-Ala-Asp-7-amino-4-Trifluoromethylcoumarin Caspase-2 Substrate (Fluorogenic) |
| 化学名 | N-acetyl-L-valyl-L-α-aspartyl-L-valyl-L-alanyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine |
| Canonical SMILES | O=C(O)CC(NC(C(NC(C(NC(C(NC(C(NC(C)=O)C(C)C)=O)CC(O)=O)=O)C(C)C)=O)C)=O)C(NC1=CC(O2)=C(C=C1)C(C(F)(F)F)=CC2=O)=O |
| 分子式 | C33H41F3N6O12 |
| 分子量 | 770.71 |
| 溶解度 | DMF: 10 mg/ml,DMSO: 10 mg/ml |
| 储存条件 | Store at -20℃ |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. |
| Shipping Condition | Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request |
| 产品描述 | Ac-VDVAD-AFC is a fluorogenic substrate whose amino acids VDVAD have been shown to be a preferred cleavage site for caspase-2.1,2 Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm. Increases in Ac-VDVAD-AFC cleavage have been reported to correlate with nitric oxide-induced activation of caspase-2 and subsequent apoptosis in human neuroblastoma lines.1
Product Description References
1. Moriya, R., Uehara, T., and Nomura, Y. Mechanism of nitric oxide-induced apoptosis in human neuroblastoma SH-SY5Y cells. FEBS Lett. 484(3), 253-260 (2000).
2. Talanian, R.V., Quinlan, C., Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).
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